Table 3.
Haplotypes | Controls | AD | DD | CD | OD |
---|---|---|---|---|---|
6 OPRD1 Tag SNPsa (2-3-6-9-10-11) | n = 429 | n = 541 | n = 245 | n = 213 | n = 103 |
TTAATA | 0.243 | 0.255 | 0.252 | 0.254 | 0.294 |
TTAACT | 0.133 | 0.115 | 0.120 | 0.119 | 0.119 |
TCCGTA | 0.122 | 0.118 | 0.117 | 0.118 | 0.093 |
TCCACT | 0.059 | 0.051 | 0.060 | 0.047 | 0.068 |
TTCATA | 0.056 | 0.056 | 0.047 | 0.055 | 0.033 |
TTAACA | 0.048 | 0.046 | 0.032 | 0.039 | 0.015 |
GCAACTb | 0.047 | 0.093 | 0.106 | 0.091 | 0.134 |
TCAATA | 0.043 | 0.041 | 0.036 | 0.034 | 0.039 |
GCAATA | 0.043 | 0.033 | 0.031 | 0.030 | 0.032 |
TCCGCT | 0.038 | 0.020 | 0.022 | 0.023 | 0.012 |
TCCATA | 0.035 | 0.028 | 0.031 | 0.036 | 0.027 |
TCAACT | 0.028 | 0.033 | 0.028 | 0.035 | 0.019 |
TCCGCA | 0.024 | 0.030 | 0.028 | 0.031 | 0.024 |
aP (by EM)c | 0.10 | 0.18 | 0.49 | 0.005 | |
All 7 OPRK1 SNPs (1-2-3-4-5-6-7) | n = 329 | n = 310 | n = 190 | n = 163 | n = 89 |
GGTTTCT | 0.448 | 0.402 | 0.399 | 0.391 | 0.411 |
GGCTTCTd | 0.186 | 0.254 | 0.231 | 0.243 | 0.235 |
GGCCCTC | 0.099 | 0.065 | 0.081 | 0.088 | 0.088 |
AGCCTCT | 0.077 | 0.071 | 0.065 | 0.064 | 0.054 |
GGCCTCT | 0.060 | 0.067 | 0.067 | 0.064 | 0.047 |
GTCCTCT | 0.051 | 0.043 | 0.043 | 0.049 | 0.033 |
GTCCTTT | 0.030 | 0.022 | 0.027 | 0.026 | 0.049 |
aP (by EM)c | 0.33 | 0.82 | 0.78 | 0.61 |
Abbreviations: AD, alcohol dependence; CD, cocaine dependence; Con, normal controls; DD, drug dependence; EM, expectation–maximization; OD, opioid dependence; SNP, single nucleotide polymorphism.
Six OPRD1 Tag SNPs were selected by Tagger in Haploview from 11 OPRD1 SNPs.
OPRD1 haplotye GCAACT was significantly more frequent in AD (χ2 = 14.82, d.f. = 1, P < 0.001), CD (χ2 = 9.19, d.f. = 1, P = 0.002) and OD (χ2 = 20.68, d.f. = 1, P < 0.001) subjects than in controls.
Global adjusted P-values were obtained by the EM algorithm.
OPRK1 haplotype GGCTTCT was significant more frequent in AD subjects than in controls (χ2 = 8.12, d.f. = 1, P = 0.004). n, number of subjects (those without genotype data for more than three OPRD1 tag SNPs were not included in OPRD1 haplotype analyses; those without genotype data for more than four OPRK1 SNPs were not included in OPRK1 haplotype analyses).
Note: Rare haplotypes (frequency < 2%) of OPRD1 and OPRK1 are not listed in the table. Statistically significant P-values are in bold.