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Minimum values of 1/(Ka1[A]) that can be used in frontal analysis experiments to produce a given deviation from linearity in double-reciprocal plots for two-site systems at various ratios for the affinities of the binding sites (β2) and at specific values for the mole fraction of the highest affinity site (α1), where α1 is equal to (a) 0.1, (b) 0.25, (c) 0.50 or (d) 0.75). These results were generated by using eq. (10).
