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. 2004 Jan;134(1):332–338. doi: 10.1104/pp.103.033126

Figure 1.

Figure 1.

Ammonium controls the light induction of ASN2. Twelve-day-old seedlings grown on Murashige and Skoog agar plates under a regular day/light cycle (16 h of light and 8 h of dark) were transferred to new Murashige and Skoog medium containing 20 mm (Lanes 1 & 2) or no (Lanes 3 & 4) ammonium. NaNO3 was added to ammonium-free medium to maintain an equal molar concentration of nitrogen resources. Plants were subsequently treated under continuous light (L) or continuous dark (D) conditions for 48 h. Total RNA was extracted from the seedlings as described in “Materials and Methods.” An aliquot of 15 μg of total RNA from each line was loaded onto each lane. Northern-blot analysis was performed as described in “Materials and Methods.”