Figure 5.

Northern-blot analysis of ASN2 transgenic lines. Twelve-day-old seedlings of the wild-type Col-0 (lanes 1 and 2), the ASN2-underexpressing line 401-A4-c1 (lanes 3 and 4), and the ASN2-overexpressing line 402-A1-b1 (lanes 5 and 6) grown on Murashige and Skoog agar plates under a regular day/light cycle were subsequently treated under continuous light (L) or continuous dark (D) conditions for 48 h. Total RNA was extracted from the seedlings as described in “Materials and Methods.” An aliquot of 15 μg of total RNA from each line was loaded onto each lane. Northern-blot analysis was performed as described in “Materials and Methods.”