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. 2004 Jan;134(1):332–338. doi: 10.1104/pp.103.033126

Figure 6.

Figure 6.

Endogenous free ammonium content decreases in ASN2-overexpressing transgenic lines grown on ammonium-containing medium. Ten-day-old seedlings of the wild-type Col-0 (lanes 1 and 6), ASN2-underexpressing lines 401-A4-c1 (lanes 2 and 7) and 401-A1-a8 (lanes 3 and 6), and ASN2-overexpressing lines 402-A1-b1 (lanes 4 and 9) and 402-IT-13 (lanes 5 and 10) grown under a regular day/light cycle on regular Murashige and Skoog (containing 20 mm ammonium) agar plates were transferred to Murashige and Skoog medium supplemented with 20 (lanes 1-5) or 50 mm (lanes 6-10) exogenous ammonium. Free ammonium content was assayed as described in “Materials and Methods.” Each bar represents an average of five samples. Error bars = ses. The data was analyzed by one-way ANOVA followed by lsd test. An asterisk indicates significant difference with a P value less than 0.05.