Figure 7.

Endogenous free ammonium content increases in ASN2 antisense transgenic lines grown under high-light irradiance. Twelve-day-old seedlings of the wild-type Col-0 (lanes 1 and 2), ASN2-underexpressing lines 401-A4-c1 (lanes 3 and 4) and 401-A1-a8 (lanes 5 and 6), and ASN2-overexpressing lines 402-A1-b1 (lanes 7 and 8) and 402-IT-13 (lanes 9 and 10) grown under a regular day/light cycle at low light irradiance (35 μE) on regular Murashige and Skoog agar plates were exposed to continuous light at low (35 μE; lanes 1, 3, 5, 7, and 9) or high (150 μE; lanes 2, 4, 6, 8, and 10) for 72 h. Free ammonium contents were assayed as described in “Materials and Methods.” Each bar represents an average of five samples. Error bars = ses. The data was analyzed by one-way ANOVA followed by lsd test. An asterisk indicates significant difference with a P value less than 0.05.