Figure 3. Cbl protection against L-Hcy-induced oxidative stress.
Pre-confluent HAEC were incubated with increasing concentrations of CNCbl for 24 h prior to the addition of 150 μM L-Hcy. ROS was measured by detecting oxidation of 3 μM DCFA. A. ROS fold-increase after 48 h. B. Cell viability after 48 h as measured with MTT assay. Data are expressed as mean ± SEM; N = 7; *p<0.05 with respect to control cells not exposed to L-Hcy; #p<0.05 with respect to L-Hcy-treated cells not exposed to CNCbl