Figure 6. Effect of Cbl on paraquat-induced O2•− levels and cell death.
HAEC were incubated in the absence or in the presence of varying concentrations of CNCbl for 24 h prior to subjection to paraquat (1.5 mM). DHE (5 μM) was added for the final 1 h of treatment. Also shown are the effects of SOD (3 μM) and apocynin (AC, 0.1 mM) on the paraquat-induced increase in O2•− and cell death. A. O2•− measured as hydroxyethidium fluorescence (λex/em = 520/605 nm) compared to untreated cells. B. Cell viability was assessed with the MTT assay. Data expressed as ± SEM; N = 3; * p < 0.05 compared to control, # p < 0.05 compared to paraquat alone.