Table IV.
Glycosyl-linkage compositions of RG-II isolated form the walls of C. thalictroides, S. molesta, sugar beet, and bamboo (Phyllostachys edulis)
RG-II was isolated by SEC from the material solubilized from C. thalictroides and S. molesta walls by treatment with EPG. The glycosyl-residue compositions were determined by GC/EI-MS analysis of the per-O-methylated alditol acetates.
Linkage | C. thalictroides | S. molesta | Sugar Beeta | Bamboob |
---|---|---|---|---|
Mol% | ||||
T-Rhapc | 7d | 6d | 5 | 5 |
2-Rhap | nde | nd | 4 | 2 |
3-Rhap | 5 | 5 | 7 | 5 |
2,3,4-Rhap | 6 | 5 | 8 | 5 |
T-Fucpf | 5 | 4 | 6 | 7 |
3,4-Fucp | 5 | 6 | 3 | 6 |
T-Arap | nd | nd | 6 | nd |
T-Araf | 4 | 5 | 5 | 6 |
2,3-Arap | 6 | 6 | nd | nd |
T-Xylpg | 3 | 4 | 7 | nd |
3′-Apif | 5 | 5 | 7 | 5 |
2,3,3′-Apif | 5 | 4 | 3 | nd |
T-Galp | 6 | 5 | 7 | 11 |
2,4-Galp | 9 | 7 | 6 | 8 |
2-GlcpA | 4 | 4 | 5 | 3 |
T-GalpA | 12 | 15 | 12 | 7 |
4-GalpA | 10 | 8 | 2 | 6 |
2,4-GalpA | nd | nd | 4 | 5 |
3,4-GalpA | 5 | 5 | 3 | 11 |
2,3,4- | 3 | 5 | nd | 8 |
GalpA |
aCalculated from Ishii and Kaneko (1998). bCalculated from Kaneko et al. (1997). cT-RhaP, 2,3,4-Tri-O-methyl-1,5-di-O-acetylrhamnitol. dIncludes terminal non-reducing 3-O-methyl rhamnosyl residues. end, Not detected. fFrom terminal non-reducing 2-O-methyl fucosyl residues. gFrom terminal non-reducing 2-O-methyl xylosyl residues.