Figure 5.

Effects of ABA, 9′-OH ABA, and neoPA on induction of elongase (FAE1) gene expression in torpedo stage microspore-derived embryos of B. napus cv Hero. Torpedo stage embryos were left untreated and harvested immediately (Control T₀ time) or treated with 10 μm ABA, 9′-OH ABA, or neoPA, for 6, 24, and 72 h. After each time point, embryos were harvested and analyzed for 3-ketoacyl-CoA synthase (FAE1) gene induction as described in “Materials and Methods.” A, Relative expression of the 3-ketoacyl-CoA synthase (FAE1) gene transcript; B, relatively equal loading of total RNA on the gel as determined by comparative intensities of the two characteristic ribosomal RNA bands.