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. Author manuscript; available in PMC: 2012 Feb 21.
Published in final edited form as: Org Biomol Chem. 2010 Dec 22;9(4):1012–1020. doi: 10.1039/c0ob00444h

Table 1.

Absorbance and fluorescence emission maxima, equilibrium dissociation constants (Kd) with scFvs either expressed on the yeast cell surface or in solution, fluorescence enhancement, and fluorescence quantum yields (Φf) for the dyes bound to soluble scFv. (Fluorescence enhancement values were calculated for soluble protein relative to the dye in buffer and were not corrected for differences in absorbance.)

scFv +
OTB-SO3 		Abs λmax
(nm)		 Em λmax
(nm)		 Yeast surface
Kd (nM)		 Protein
Kd (nM)		 Fluor.
Enhance.		 Φf
A5 411 431 109.5 ± 5.5 93.0 ± 2.9 52.6 0.841
A6 400 422 6.9 ± 1.4 9.4 ± 0.5 9.73 0.047
B11 400 424 13.0 ± 0.8 7.7 ± 0.3 20.2 0.353
D10 410 426 12.3 ± 0.7 12.9 ± 0.8 49.2 0.904
H10 405 440 119.0 ± 6.5 72.4 ± 4.1 54.5 1.00
J10 410 435 166.9 ± 4.2 63.3 ± 2.0 50.7 0.877
scFv + t-butyl-OTB-SO3
A5 413 434 42.3 ± 1.4 61.5 ± 3.1 36.6 0.827
scFv + t-butyl-OTB-CO2
A5 413 437 39.4 ± 1.5 31.2 ± 1.8 40.0 0.605
scFv + DIR
J10 625 650 24.4 ± 1.6 21.2 ± 1.0 8.2 0.257