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. Author manuscript; available in PMC: 2011 Aug 28.
Published in final edited form as: Cell Signal. 2010 Jan 13;22(5):821–827. doi: 10.1016/j.cellsig.2010.01.006

Fig. 1.

Fig. 1

Expression and distribution of LRRK2. (A) A LRRK2-specific rabbit polyclonal antibody was raised against the region 800–1000 amino acids (MID) between the ANK and LRR domains of human LRRK2. Location of the mutations used in the study is shown in red. R1441C in the COR domain and G2019S in the kinase domain are PD-associated mutations, whereas K1906N is a synthetic mutant with an impaired kinase activity. (B) Extracts of HEK293 cells transfected for 48 h with [wt]LRRK2 were run on a 6% polyacrylamide gel, immunoblotted with the antibody against LRRK2-MID (lower panel) and reprobed with an antibody against the HA tag (upper panel). LRRK2 protein is detected at approximately 280 kDa by both HA-specific and LRRK2-specific antibodies. The LRRK2 antibody detected also endogenous LRRK2 in extracts from mock-transfected cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)