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. Author manuscript; available in PMC: 2011 Aug 28.
Published in final edited form as: Cell Signal. 2010 Jan 13;22(5):821–827. doi: 10.1016/j.cellsig.2010.01.006

Fig. 3.

Fig. 3

Time course of ERK1/2 activation in inducible cell lines. [wt]LRRK2 and [R1441C] LRRK2 inducible HEK293 cells were treated with tetracycline for the indicated times and subjected to immunoblot analysis with antibodies against phospho- and total ERK1/2. Relative densitometry intensities for phospho-ERK1/2 immunoreactivity showed that over-expression of the PD-causative mutation R1441C led to a delayed activation of ERK1/2 compared to [wt]LRRK2. Each value is averaged from 4 independent experiments.