Table 1.

Design of molecular beacons for detection of e and survivin genes and corresponding target sequences xpression of mutant K-ras

MBs	Target sequences	Design of the MBs
K-ras MB1	K-ras codon 12 GGT to GAT mutation	5′-Cy 3-CCTACGCCATCAGCTCCGTAGG-Dabcyl-3′
K-ras MB2	K-ras codon 12 GGT to GTT mutation	5′-Texas-red-CCTACGCCAACAGCTCCGTAGG-Dabcyl-3′
Survivin MB1	Survivin cDNA from 27 to 43 nucleotide	5′-Cy3-CTGAGAAAGGGCTGCCAGTCTCAG-Dabcyl-3′
Survivin MB2	Survivin cDNA from 33 to 50 nucleotide	5′-FITC-TGGTCCTTGAGAAAGGGCGACCA-Dabcyl-3′
GAPDH MB	GAPDH cDNA from 504 to 521 nucleotide	5′-Cy3-GAGTCCTTCCACGATACCGACTC-Dabcyl-3′
DNA Targets	Synthesized Oligonucleotide sequences
K-ras WT (GGT)	5′-GTA GTT GGA GCT GGT GGC GTA GGC AAG AGTGCCTTGACGATACAGCTAATT CAG-3′
K-ras Mut 1 (GAT)	5′-GTA GTT GGA GCT GAT GGC GTA GGC AAG AGTGCCTTGACGATACAGCTAATT CAG-3′
K-ras Mut 2 (GTT)	5′-GTA GTT GGA GCT GTT GGC GTA GGC AAGAGTGCCTTGACGATACAGCTAATT CAG-3′
Survivin	5′-CCTGCCTGGCAGCCCTTTCTCAAGGACCACCGCATCTCTACATTCAAGAAC-3′