Table 2.
Number (#) and mutant frequencies (MF) of sequenced transitions and transversions in the rpoB gene in mismatch-repair-proficient polA+ (wild-type) and polAexo strains.
| Class of mutation | rpoB position | Wild-type
|
polAexo |
||
|---|---|---|---|---|---|
| # | MF | # | MF | ||
| Base substitutions | 181 | (1.86) | 207 | (3.4) | |
| Transitions | 107 | (1.09) | 120 | (1.97) | |
| A·T → G·C | 29 | (0.298) | 50 | (0.821) | |
| 1532 | 3 | (0.030) | 4 | (0.065) | |
| 1534 | 6 | (0.061) | 18 | (0.295) | |
| 1538 | 1 | (0.010) | 4 | (0.065) | |
| 1547 | 15 | (0.154) | 21 | (0.344) | |
| 1598 | 4 | (0.041) | 3 | (0.049) | |
| G·C → A·T | 78 | (0.801) | 70 | (1.149) | |
| 1535 | 8 | (0.082) | 0 | (0.000) | |
| 1546 | 8 | (0.082) | 16 | (0.262) | |
| 1565 | 1 | (0.010) | 0 | (0.000) | |
| 1576 | 24 | (0.246) | 21 | (0.345) | |
| 1586 | 3 | (0.030) | 0 | (0.000) | |
| 1592 | 21 | (0.216) | 21 | (0.345) | |
| 1691 | 13 | (0.133) | 12 | (0.197) | |
| Transversions | 74 | (0.760) | 87 | (1.428) | |
| G·C → T·A | 18 | (0.185) | 23 | (0.377) | |
| 436 | 2 | (0.020) | 4 | (0.065) | |
| 1535 | 0 | (0.000) | 2 | (0.033) | |
| 1537 | 1 | (0.010) | 2 | (0.033) | |
| 1546 | 6 | (0.061) | 4 | (0.065) | |
| 1576 | 7 | (0.072) | 9 | (0.148) | |
| 1592 | 2 | (0.020) | 2 | (0.033) | |
| A·T → T·A | 42 | (0.431) | 52 | (0.854) | |
| 1538 | 22 | (0.226) | 26 | (0.427) | |
| 1547 | 2 | (0.020) | 3 | (0.049) | |
| 1577 | 13 | (0.133) | 13 | (0.213) | |
| 1598 | 0 | (0.000) | 2 | (0.033) | |
| 1714 | 5 | (0.051) | 8 | (0.131) | |
| A·T → C·G | 9 | (0.092) | 7 | (0.115) | |
| 1538 | 3 | (0.030) | 2 | (0.033) | |
| 1714 | 6 | (0.061) | 2 | (0.033) | |
| 1715 | 0 | (0.000) | 3 | (0.049) | |
| G·C → C·G | 5 | (0.051) | 5 | (0.082) | |
| 1576 | 5 | (0.051) | 5 | (0.082) | |
Values within parentheses indicate the frequencies (per 108 cells) of individual or classes of mutations. These frequencies were calculated by multiplying their number relative to the total number of sequenced mutations by the overall mutant frequencies of Table 1.