Table 3.
Number (#) and mutant frequencies (MF) of sequenced base-pair substitutions in the rpoB gene for polA+ and polAexo strains in the mismatch-repair-deficient mutL background.a
| Class of Mutation | rpoB position |
mutL |
mutL polAexo |
mutL dnaE915 |
mutL dnaE915polAexo |
||||
|---|---|---|---|---|---|---|---|---|---|
| # | (MF) | # | (MF) | # | (MF) | # | (MF) | ||
| Base substitutions | 359 | (311) | 360 | (321) | 179 | (81) | 175 | (121) | |
| Transitions | 339 | (293) | 353 | (315) | 173 | (78) | 165 | (114) | |
| A·T → G·C | 212 | (184) | 224 | (200) | 76 | (34.4) | 92 | (63.6) | |
| 1532 | 27 | (23.4) | 27 | (24.1) | 8 | (3.62) | 6 | (4.14) | |
| 1534 | 30 | (26.0) | 32 | (28.5) | 32 | (14.5) | 49 | (33.9) | |
| 1538 | 37 | (32.1) | 37 | (33.0) | 9 | (4.07) | 11 | (7.6) | |
| 1547 | 118 | (102) | 128 | (114) | 26 | (11.8) | 24 | (16.6) | |
| 1552 | 0 | (0.0) | 0 | (0.0) | 0 | (0.0) | 1 | (0.69) | |
| 1598 | 0 | (0.0) | 0 | (0.0) | 1 | (0.45) | 1 | (0.69) | |
| G·C → A·T | 127 | (110) | 129 | (115) | 97 | (43.9) | 73 | (50.5) | |
| 1535 | 1 | (0.86) | 1 | (0.89) | 0 | (0.0) | 1 | (0.69) | |
| 1546 | 55 | (47.6) | 59 | (52.6) | 75 | (33.9) | 46 | (31.8) | |
| 1576 | 25 | (21.7) | 32 | (28.5) | 4 | (1.81) | 4 | (2.76) | |
| 1586 | 8 | (6.93) | 8 | (7.13) | 10 | (4.52) | 16 | (11.1) | |
| 1592 | 11 | (9.53) | 7 | (6.24) | 3 | (1.35) | 3 | (2.07) | |
| 1691 | 27 | (23.4) | 22 | (19.6) | 5 | (2.26) | 3 | (2.07) | |
| Transversions | 20 | (17.32) | 7 | (6.24) | 6 | (2.71) | 10 | (6.91) | |
| G·C → T·A | 3 | (2.59) | 2 | (1.78) | 4 | (1.81) | 8 | (5.53) | |
| 436 | 1 | (0.86) | 0 | (0.0) | 0 | (0.0) | 0 | (0.0) | |
| 1546 | 0 | (0.0) | 0 | (0.0) | 0 | (0.0) | 1 | (0.69) | |
| 1576 | 2 | (1.73) | 1 | (0.89) | 3 | (1.35) | 3 | (2.07) | |
| 1592 | 0 | (0.0) | 1 | (0.89) | 1 | (0.45) | 4 | (2.76) | |
| A·T → T·A | 16 | (13.9) | 5 | (4.45) | 2 | (0.90) | 2 | (1.38) | |
| 1538 | 1 | (0.86) | 0 | (0.0) | 0 | (0.0) | 1 | (0.69) | |
| 1577 | 1 | (0.86) | 1 | (0.89) | 0 | (0.0) | 0 | (0.0) | |
| 1598 | 3 | (2.59) | 0 | (0.0) | 0 | (0.0) | 0 | (0.0) | |
| 1714 | 11 | (9.53) | 4 | (3.56) | 2 | (0.90) | 1 | (0.69) | |
| A·T → C·G | 1 | (0.86) | 0 | 0 | 0 | ||||
| 1715 | 1 | (0.86) | 0 | (0.0) | 0 | (0.0) | 0 | (0.0) | |
a
Values within parentheses indicate the frequencies (per 108 cells) of individual or classes of mutations (see legend to Table 2).