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. 2011 Aug 8;9:31. doi: 10.1186/1477-3155-9-31

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Copyright ©2011 Nejadmoghaddam et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Specific binding capacity of three Nickel-magnetic matrices for two His-tagged recombinant proteins. After binding of His-tagged recombinant proteins, His-ProT and His-Mre11, onto the Nickel-magnetic matrices, SiMAC-Nickel, SiMAG/N-NTA/Nickel and SiMAG/CS-NTA/Nickel, flowthrough fractions (FT) were subjected to SDS-PAGE analysis. Percent of band density in FT subtracted from 100% was defined as specific binding capacity. His-ProT (1), His-Mre11 (2), SiMAC-Nickel (A), SiMAG/N-NTA/Nickel (B) and SiMAG/CS-NTA/Nickel (C).