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Hyaluronan impairs oligodendrocyte maturation in organotypic slice cultures. (A) Density of MBP+ oligodendrocytes in the white matter at 9 DIV in PBS (Black bar) and HA (white bar) treated slices. Data are mean ± SEM (n = 9 slices per time group from three independent experiments). *P < 0.0005 (unpaired two-way t-test). (B, C) Representative photomicrographs of 9 DIV slices stained with MBP in the PBS (B) and HA (C) treatment groups. Scale: B, C, 100 μm.
