Table I.
Transgenic plants and unusual fatty acid composition
| Plant Lines | Transgenes | Unusual Fatty Acidsa | ||||
|---|---|---|---|---|---|---|
| RAd | DAe | LAf | VAg | Epox. FAh | ||
| mol% | ||||||
| N10.3pC3.1 | Napin-PHA synthase + vector pCAMBIA 1300 | ndi | nd | nd | nd | nd |
| N10.3NH1.1 | Napin-PHA synthase + napin-hydroxylase | 1.2 ± 0.2 | 1.6 ± 0.1 | 0.7 ± 0.1 | nd | nd |
| N10.3NH2.2 | Napin-PHA synthase + napin-hydroxylase | 3.0 ± 0.1 | 2.4 ± 0.1 | 0.9 ± 0.1 | nd | nd |
| N10.3NE6.2 | Napin-PHA synthase + napin-epoxygenase | nd | nd | nd | 0.9 ± 0.1 | nd |
| N4.1pC6.1 | Napin-PHA synthase + vector pCAMBIA 1300 | nd | nd | nd | nd | nd |
| N4.1NE15.1 | Napin-PHA synthase + napin-epoxygenase | nd | nd | nd | 2.9 ± 0.1 | 0.2 ± 0.1 |
| WTPC7.3 | Vector pCAMBIA 1300 | nd | nd | nd | nd | nd |
| WTNH8.1 | Napin-hydroxylase | 4.8 ± 0.1 | 3.5 ± 0.1 | 1.1 ± 0.1 | nd | nd |
| WTNH13.1 | Napin-hydroxylase | 5.1 ± 0.2 | 3.4 ± 0.4 | 1.1 ± 0.1 | nd | nd |
| WTNE5.1 | Napin-epoxygenase | nd | nd | nd | 2.8 ± 0.1 | 0.2 ± 0.1 |
| WTNE7.4 | Napin-epoxygenase | nd | nd | nd | 2.7 ± 0.4 | 0.1 ± 0.1 |
| PHAC3.3b | CaMV 35S-PHA synthase | |||||
| N-FatB3c | Napin-caproyl-ACP thioesterase | |||||
a Values presented are means of three values ± sd. b Line previously described by Mittendorf et al. (1998). c Line previously described by Poirier et al. (1999). d 12-Hydroxy-9-octadecenoic acid (ricinoleic acid). e 12-Hydroxy-9, 15-octadecadienoic acid (densipolic acid). f 14-Hydroxy-11-dodecenoic acid (lesquerolic acid). g 12-Epoxy-9-octadecenoic acid (vernolic acid). h 12-Epoxy-9, 15-octadecadienoic acid. i Not detected.