NEC5 expression patterns. Whole RNA was isolated from the indicated tissues, and RT-PCR was performed as in “Materials and Methods.” A, Temporal expression of nec5 transcript in ornamental tobacco nectaries. The stage of the tissue that was the source of the RNA are: lane 1, stage 2; lane 2, stage 6; lane 3, stage 8; lane 4, stage 10; lane 5, stage 12; lane 6, nectary tissues post-fertilization. B, Spatial expression of nec5 transcript in ornamental tobacco tissues. The floral organ or vegetative tissue that was the source of RNA are: lane 1, nectary; lane 2, ovary; lane 3, style; lane 4, stigma; lane 5, floral tube; lane 6, anther/filament; lane 7, petal; lane 8, sepal; lane 9, receptacle; lane 10, leaf; lane 11, stem; lane 12, root.