Table I.

Oligonucleotides used in this study

Oligo Name	Sequencea	Purpose
Nec5-5′	GCN-GTN-CAR-AAR-AAR-TT	Degenerate RT-PCR
Nec5-3′	GTN-GTR-TAD-ATN-GGR-AA	Degenerate RT-PCR
NecV-INV1	TTA-GTG-ACC-AAA-AAT-TCC-CC	First round inverse-PCR
NecV-INV 2	CAG-ATA-AGC-ATT-GAA-GGA-AC	First round inverse-PCR
NecV-FOR1	CAA-ATG-GGA-CGG-GAA-AAA-TGT-G	Second round inverse-PCR
NecV-REV1	GAT-CCT-CTG-ATA-CAT-AAG-AAA-GGC	Second round inverse-PCR
N5-FOR2	CTC-AGT-ACA-ACA-ACT-AGA-AGG	Internal Primer for RT-PCR
N5-REV	GAG-GAA-TAT-AGA-GAC-GAA-TG	Internal primer for RT-PCR

^an = G + A + T + C; R = G + A; D = A + T + C.