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The −205/−175 region of the Mcl-1 promoter is essential for transcriptional upregulation of Mcl-1 in melanoma cells under ER stress. (a) A schematic illustration of construction of the luciferase reporter constructs. (b) Mel-CV (left) and MM200 (right) cells were transiently transfected with indicated pGL3-basic based reporter constructs. After 24 h, cells were treated with TM (3 μ) for a further 16 h followed by measurement of the luciferase activity. Bars, s.e. (n=3).
