Fig. 2.
CR effects on silencing of a Ty1-mURA3 marker within the tandem array. Strains with Ty1-mURA3 integrated within the tandem array at NTS2 (JS125) and NTS1 (JS128), or at a non-rDNA genomic location (JS122) were spotted as 5-fold serial dilutions onto SC and SC-ura plates containing 2%, 0.5%, 0.2%, or 0.05% glucose. The sir2Δ control strains were JS151 (non-rDNA), JS155 (NTS2), and JS163 (NTS1). Plates were incubated for 3 days prior to photography.