Tab. 5.
Factors and levels evaluated in the robustness assays.
| Factor | ID | Factor classific. | Limit | Low Level (−) | High Level (+) | Nominal Level |
|---|---|---|---|---|---|---|
| Cell Culture Media manufacturer | G | qual. | – | GIBCO | PAA | GIBCO |
| Fetal Bovine Sera manufacturer | E | qual. | – | GIBCO | PAA | GIBCO |
| Time of Trypsinization | I | quant. | ±1 min | 11 min (A431) 2 min (H125) |
13 min (A431) 4 min (H125) |
12 min (A431) 3 min (H125) |
| Trypsin-EDTA manufacturer | B | qual. | – | GIBCO | PAA | GIBCO |
| Incubation time for EGFR recovery | A | quant. | ±10 min | 50 min | 70 min | 60 min |
| Incubation time of mAb | C | quant. | ±5 min | 25 min | 35 min | 30 min |
| Incubation time of FITC-conjugated | H | quant. | ±5 min | 25 min | 35 min | 30 min |
| Volume of mAb | D | quant. | ±5 μL | 15 μL | 25 μL | 20 μL |
| Volume of FITC-conjugated | K | quant. | ±5 μL | 15 μL | 25 μL | 20 μL |
| Formaldehyde cell fixation | J | qual. | – | No | Yes | No |