Figure 3.

Nanogel-coated surfaces show resilience to a harsh surfactant environment compared with BSA-coated surfaces. Coated surfaces were exposed to antibody, washed with sodium dodecyl sulfate (SDS), re-exposed to antibody and re-washed with SDS. Adsorption measurements were obtained in between each step. (a) Raw TIRF images of (top row) a covalently coupled BSA-coated surface and (bottom row) a nanogel-coated surface over the course of a five-step antibody adsorption experiment. (1) Surfaces prior to antibody exposure. (2) Surfaces after exposure to antibody. (3) Surfaces after SDS wash. (4) Surfaces after second exposure to antibody. (5) Surfaces after second SDS wash. (b) The bar chart depicts the ratio of adsorption change after SDS exposure to adsorption change before SDS exposure, i.e. (step 4 – step 3) : (step 2 – step 1). Error bars represent standard deviation of duplicate substrates.