Figure 4.

Nanogel coatings can be utilized for sensitive and specific, digital antibody binding. (a) Cy3-target proteins were immobilized to a nanogel-coated surface and then bound by Cy5-antibody. (b) Merged raw image of red and green TIRF channels (15 µm × 15 µm). (c) Merged red and green TIRF channels after image processing. TIRF imaging was able to detect a high number of binding events (yellow) in which target proteins (green) were accessible to binding by antibodies (red). (d) The correlation between detection antibodies and individual analyte proteins tethered to nanogel-coated surfaces indicates high target protein accessibility and compatibility with TIRF detection.