Figure 2.

cic acts downstream of the Tor pathway and is not required for Gro activity. (A) Diagram of the Tor cascade. (B,C) Cuticle phenotypes of embryos derived from doubly homozygous tor; cic females (B) and from cic¹ females carrying germ-line clones of Draf (C); the embryos display a cic-like phenotype, suggesting that cic acts downstream of Draf. The roles of cic, tor, and Draf in terminal patterning are strictly maternal and the mutations do not show paternal rescue. (D,E) Antibody staining for activated Erk protein in wild-type (D) and cic¹ (E) embryos; the similar staining indicates that the domain of Tor signaling is unaffected in cic¹ mutant embryos. (F–H) Effects on Sex-lethal (Sxl) expression of ectopic hairy (h) activity driven by the hunchback (hb) promoter in otherwise wild-type (F), gro^E48 (G), and cic¹ (H) embryos. Embryos were stained with an antibody against the active Sxl protein. Repression of Sxl by Hairy requires gro, however the Hairy/Gro complex does not require cic function.