Figure 8.
SELDI-TOF MS ProteinChip immunoassays of amniotic fluid. (a) The IgG negative control for group 1 (preterm labor without infection/inflammation who deliver at term) (b) The IGFBP-1 capture antibody assay for the same amniotic fluid. (c) The IgG negative control for group 3 (preterm labor with intra-amniotic infection/inflammation). (d) The IGFBP-1 capture antibody assay for the same amniotic fluid (group 3). Peaks near 13 kDa correspond to proteolytic fragments of IGFBP-1, while the peak near 25 kDa correspond to the intact IGFBP-1 protein. Comparison of Figures (b) and (d) reveals evidence for proteolytic cleavage occurring in amniotic fluid from women with intra-amniotic infection/inflammation (group 3), but not in those without it (group 1).