Figure 2.
Representative Mcl-1 RNA in situ hybridization results on preimplantation embryos. (a) Light- and dark-field images of a morula stage wild-type embryo hybridized with the Mcl-1 anti-sense probe. (b) Light- and dark-field images of a blastocyst stage wild-type embryo with the Mcl-1 antisense probe revealing signal in both TE and ICM. (c) The Mcl-1 sense probe hybridized to wild-type blastocyst displayed no signal above background. (d) Light- and dark-field image of an Mcl-1 null blastocyst hybridized with the Mcl-1 antisense probe. (e) Representative noncompacted blastomere stage Mcl-1 null embryo that fails to hybridize with the Mcl-1 antisense probe. Magnification, 400×.