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. 2011 Jul 27;31(30):10948–10970. doi: 10.1523/JNEUROSCI.0323-11.2011

Figure 2.

Figure 2.

Time course of MGE- and CGE-derived interneuron migration to the hippocampus from the ganglionic eminences. A–F, Representative images illustrating the migration of GFP+ CGE-derived interneurons generated on E12.5 in the Mash1CreER:RCE line. The time points indicate the duration between tamoxifen administration (E12.5 for all panels) and when tissue was collected. Sections were counterstained with DAPI (blue). The yellow arrowhead indicates the hippocampal anlage (HP). The migration from their site of genesis on E12.5 in the CGE to hippocampus lasts ∼72 h. Scale bar: (in D) 200 μm (all panels). G, H, Histograms illustrating the normalized density of CGE- and MGE-derived hippocampal interneurons from the Mash1CreER:RCE and Olig2CreER:ZEG lines for tamoxifen administrations between E10.5 and E16.5 as indicated. Data are normalized to the peak density observed for all tamoxifen injection time points within each mouse line (E14.5 + 96 h for Mash1CreER:RCE and E12.5 + 48 h for Olig2CrER:ZEG). The duration of migration for interneurons generated later in development is shorter than those born early despite the fact that the enlarging brain has resulted in a longer path of migration. Total number of cells counted (from left to right) were as follows: for the Mash1CreER:RCE, n = 0, 2248, 2713, 2419, 1746, 988, 3516, 3016, 5144, 3402, 1888, 1462, 2282, 2896, 2340, 1455, 1072, 583; and for the Olig2CreER:ZEG, n = 0, 0, 146, 151, 73, 44, 211, 174, 156, 94, 73, 59.