Figure 7.

Representative MGE-derived hippocampal interneurons. A–K, Neurolucida reconstructions of GFP+ interneurons recorded in slices from P15–P30 Nkx2-1Cre:RCE pups (dendrites and soma in black; axon in red). Scale bar: 100 μm. The dashed lines indicate the approximate boundaries of s.o., s.p., s.r., and s.l.m. Under each drawing is shown the molecular profile obtained from single-cell PCR analysis for the recorded cell with filled boxes indicating transcripts detected. Also shown are the electrophysiological responses of the cells to the indicated square wave current pulses (bottom) from a resting potential near −60 mV. Depolarizing current pulses and corresponding responses are for near threshold and 2× threshold stimulation (scale bars shown in K are for all traces). Phase plots of the APs arising from 2× threshold stimulation are shown at right, with the first AP phase plot colored red and subsequent APs progressing from warm to cool colors ending in violet. L, Histogram summarizing the frequency of occurrence for the 16 transcripts probed by scPCR among the MGE cohort of recorded cells.