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. 2000 Jan 15;14(2):232–244.

Figure 1.

Figure 1

Impaired differentiation and increased cell death in dnStat5-expressing tsE26 myeloblasts after shift to 42°C. (A, left) Stat5dC and Stat5Y-F function as dominant-negative Stat5 versions. Clones verified for high dnStat5 expression by Western blot were transfected with a reporter construct containing three IFP-53 GAS sites (top) as described earlier (Woldman et al. 1997). (Right) Northern blot analysis for CIS mRNA expression, using a control clone and a Stat5dC-expressing clone after stimulation with cMGF. (B–D) Cells from two representative clones expressing empty vector (B), Stat5dC (C), or Stat5Y-F (D) were induced to differentiate at 42°C in the presence of cMGF and IGF-1 for 4 days, cytocentrifuged onto slides, stained with May-Gruenwald-Giemsa solution, and photographed. Note terminally differentiated macrophages with a highly excentric nucleus in B but dead cells (C) or small, partially differentiated cells (D) in the cultures expressing dominant-negative versions of Stat5.