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. 2000 Feb 1;14(3):278–288.

Figure 3.

Figure 3

ATM dependence of p53 phosphorylation on Ser-20 in response to IR, but not UV light. Primary fibroblasts from a normal individual (AG1522) or from A-T patients (AT1BR and AT5BI) were exposed to IR or UV light. Phosphorylation of p53 on Ser-20 (S20p) was monitored with the phosphate-specific antibody AbS20p. p53* indicates that to facilitate analysis of the p53 phosphorylation state, the amounts of extract used in each lane of the top and bottom panels were adjusted so that all lanes had equal levels of total p53 (bottom; immunoblotting with antibody DO7).