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. 2011 Aug 29;6(8):e23516. doi: 10.1371/journal.pone.0023516

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The figure shows X-gal staining of osteoblasts isolated from femurs and tibia of a wild type (Cx43^flx/flx, WT) and a Cx43 deficient (OC-Cre;Cx43^flx/flx, KO) mouse. Cells from wild type mice had no blue staining indicating that floxed Cx43 was not deleted in the absence of OC-Cre. Cells from a Cx43 deficient mouse were positive for β-galactodase indicating Cx43 deleted by Cre.