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. 2011 Aug 29;6(8):e23573. doi: 10.1371/journal.pone.0023573

Figure 1. Injection of ccc-plasmids into the cytoplasm of a zygote.

Figure 1

A) Schematic depiction of cytoplasmic plasmid injection (CPI) into an opaque zygote. B) Cytoplasmic injection into a porcine zygote. C) For comparative reasons a pronuclear injection in a porcine zygote is shown. To reveal the pronuclei, a high speed centrifugation at 12'000–15'000 g is necessary.