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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Pharm Sci. 2011 Mar 17;100(8):3396–3404. doi: 10.1002/jps.22527

Fig. 1.

Fig. 1

SPR sensorgrams of AT III-heparin and human thrombin-heparin interaction and IC50 measurement of heparin product with different autoclaving time using surface competition SPR. Panel A. Sensorgrams of heparin-AT III interactions. Concentrations of AT III (from top to bottom): 1000, 500, 250, 125, and 63 nM, respectively. The black curves in all sensorgrams are the fitting curves using models from BIAevaluate 4.0.1. Panel B. Competition SPR sensorgrams of AT III-heparin interaction (solution heparin/surface heparin competition). AT III concentration was 250nM, and concentrations of heparin in solution (from top to bottom) were 0,1.25, 2.5, 5, 10 U/ml, respectively; Panel C. IC50 measurement of heparin product with different autoclaving time to AT III using surface competition SPR. Color code: red: non-sterilized heparin, blue: 30 min sterilized heparin, pink: 60 min sterilized heparin, and green: 120 min sterilized heparin. Panel D. Sensorgrams of heparin-human factor IIa (thrombin) interactions. Concentrations of human thrombin (from top to bottom): 1000, 500, 250, 125, and 63 nM, respectively. The black curves in all sensorgrams are the fitting curves using models from BIAevaluate 4.0.1. Panel E. Competition SPR sensorgrams of human thrombin-heparin interaction (solution heparin/surface heparin competition). Human thrombin concentration was 125nM, and concentrations of heparin in solution (from top to bottom) were 0, 0.63, 1.25, 2.5, 5 U/ml, respectively; Panel F. IC50 measurement of heparin product with different autoclaving time to human thrombin using surface competition SPR. Color code: red: non-sterilized heparin, blue: 30 min sterilized heparin, pink: 60 min sterilized heparin, and green: 120 min sterilized heparin.