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PAGE analysis of heparin product with different autoclaving time. A. Lane 1, HP oligosaccharide standards; lane 2 to 4: non-sterilized heparin, 30 min sterilized heparin, 60 min sterilized Heparin, and 120 min sterilized heparin. B. Molecular weight change of heparin product with different autoclaving time. The PD values for the four samples were 1.15, 1.16, 1.20, 1.22, respectively.
