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600 MHz 1H-NMR spectra of non-sterilized heparin (A), 30 min sterilized heparin (B), 60 min sterilized heparin (C), 120 min sterilized heparin (D) recorded in D20 at 298 K. Signals, a, H1 GlcNS, GlcNS6S; b, H1 IdoA2S; c, H1 IdoA; d, H1 GlcA; e, H6 GlcNS6S; f, H2 Ido2S; g, H6′ GlcNS6S; h, H3 IdoA2S; i, H4 IdoA2S; j, H5 GlcNS6S; k, H6 GlcNS; l, H4 GlcNS6S; m, H3 GlcNS, GlcNS6S; n, H2 GlcA; o, H2 GlcNS6S; p, H2 GlcNH2; q, acetyl CH3; Asterisks shows undefined peaks.
