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. Author manuscript; available in PMC: 2011 Aug 29.
Published in final edited form as: J Urol. 2010 Jun 19;184(2):769–774. doi: 10.1016/j.juro.2010.03.110

Figure 4.

Figure 4

Detailed structures of the urethral musculature. At 1000x magnification, PDE5 staining (green) revealed the characteristic striations of striated muscles, as demonstrated clearly in A. The same picture (A) also shows the SMA (red)-stained fibers are sandwiched between the much larger striated fibers. Pictures B and C show the intermingling striated and smooth muscle fibers on the outer and inner rims, respectively, of the circular striated muscle layer. DAPI staining (blue) serves to locate cellular nuclei. Note that the intermingled fiber centrally located in B can also be seen at the 200x magnification in Fig. 2 (labeled by “sm?”). In this case, the absence of DAPI staining allows better visualization of the green color within the red color.