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. Author manuscript; available in PMC: 2011 Aug 29.
Published in final edited form as: J Sex Med. 2010 Apr 1;7(5):1787–1797. doi: 10.1111/j.1743-6109.2010.01749.x

Fig 3. PTX decreases phosphorylation of the Smad1(5)/Smad6 pathway.

Fig 3

TADF cells were treated with vehicle, TGF-β1 (1ng/ml), or the combination of TGF-β1 and PTX (TGF-β1 followed by PTX, or PTX followed by TGF-β1) for 24 hr. A. Western blot of pSmad1/5, Samd1/5, pSmad2, and Smad2, in TADF cells. All TGF-β1 treatments increased phosphorylation of both Smad 1/5 and Smad2 ( # P<0.01). B. Phosphorylation level of Smad1/5 in TADF. Pretreatment with PTX significantly decreased the phosphorylation level of Smad1/5 (*P<0.01). C. Phosphorylation level of Smad2 in TADF Pretreatment with PTX did not affect the phosphorylation level of Smad2 (#p>0.05).