Table 1.
A | B | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
MLcon | MLalp | MLcon | MLalp | |||||||||||||
FAME [%] | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||||||||
C14:0 | 12.32 | ± | 0.51 | b | 9.67 | ± | 0.25 | d | 13.09 | ± | 0.18 | a | 10.21 | ± | 0.18 | c |
C14:1 c9 | 1.04 | ± | 0.05 | b | 0.89 | ± | 0.03 | d | 1.15 | ± | 0.02 | a | 0.98 | ± | 0.01 | c |
C15:0 | 1.24 | ± | 0.05 | d | 1.35 | ± | 0.02 | c | 1.47 | ± | 0.04 | b | 1.58 | ± | 0.04 | a |
C16:0 | 38.36 | ± | 1.29 | b | 26.49 | ± | 0.89 | d | 43.50 | ± | 1.35 | a | 29.76 | ± | 0.53 | c |
C16:1 c9 | 1.70 | ± | 0.05 | b | 1.37 | ± | 0.04 | d | 1.97 | ± | 0.01 | a | 1.62 | ± | 0.04 | c |
C17:0 | 0.56 | ± | 0.02 | c | 0.68 | ± | 0.01 | b | 0.68 | ± | 0.02 | b | 0.79 | ± | 0.01 | a |
C18:0 | 9.53 | ± | 1.07 | 10.19 | ± | 0.85 | 9.92 | ± | 0.22 | 10.54 | ± | 0.26 | ||||
C18:1 c9 | 14.06 | ± | 0.65 | d | 19.79 | ± | 0.68 | b | 15.56 | ± | 0.19 | c | 21.71 | ± | 0.27 | a |
C18:1 c11 | 0.46 | ± | 0.02 | d | 0.60 | ± | 0.02 | b | 0.50 | ± | 0.02 | c | 0.66 | ± | 0.02 | a |
C18:1 c12-15 | 0.40 | ± | 0.02 | b | 0.55 | ± | 0.03 | a | 0.41 | ± | 0.03 | b | 0.56 | ± | 0.05 | a |
C18:1 t4-8 | 0.09 | ± | 0.01 | b | 0.25 | ± | 0.02 | a | 0.09 | ± | 0.02 | b | 0.25 | ± | 0.01 | b |
C18:1 t9 | 0.16 | ± | 0.01 | b | 0.29 | ± | 0.01 | a | 0.15 | ± | 0.03 | b | 0.30 | ± | 0.01 | a |
C18:1 t10 | 0.21 | ± | 0.02 | c | 0.28 | ± | 0.02 | b | 0.22 | ± | 0.02 | c | 0.32 | ± | 0.01 | a |
C18:1 t11 (VA) | 0.47 | ± | 0.02 | c | 4.54 | ± | 0.13 | b | 0.51 | ± | 0.01 | c | 4.96 | ± | 0.05 | a |
C18:1 t12-16 | 0.81 | ± | 0.07 | c | 1.25 | ± | 0.10 | b | 0.91 | ± | 0.03 | c | 1.44 | ± | 0.04 | a |
C18:2 n-6 | 1.06 | ± | 0.09 | b | 1.30 | ± | 0.06 | ab | 1.48 | ± | 0.43 | ab | 1.73 | ± | 0.38 | a |
C18:2 n-6 (t,t) | 0.30 | ± | 0.02 | c | 0.52 | ± | 0.04 | b | 0.35 | ± | 0.03 | c | 0.71 | ± | 0.06 | a |
C18:2 c9,t11 | 0.31 | ± | 0.03 | c | 2.77 | ± | 0.13 | b | 0.32 | ± | 0.04 | c | 3.04 | ± | 0.08 | a |
C18:2 t11,c13 | 0.00 | ± | 0.00 | c | 0.25 | ± | 0.01 | b | 0.00 | ± | 0.00 | c | 0.29 | ± | 0.02 | a |
C18:2 t9,t11 | 0.03 | ± | 0.01 | c | 0.12 | ± | 0.00 | b | 0.01 | ± | 0.01 | d | 0.16 | ± | 0.02 | a |
C18:2 t11,t13 | 0.00 | ± | 0.00 | c | 0.05 | ± | 0.00 | a | 0.00 | ± | 0.00 | c | 0.04 | ± | 0.01 | b |
C18:3 n-3 | 0.34 | ± | 0.03 | d | 1.27 | ± | 0.06 | b | 0.43 | ± | 0.03 | c | 1.46 | ± | 0.02 | a |
C20:0 | 0.21 | ± | 0.11 | 0.17 | ± | 0.02 | 0.20 | ± | 0.04 | 0.19 | ± | 0.03 | ||||
C20:1 c9 | 0.02 | ± | 0.00 | 0.03 | ± | 0.01 | 0.04 | ± | 0.02 | 0.03 | ± | 0.02 | ||||
C20:3 n-6 | 0.09 | ± | 0.01 | 0.07 | ± | 0.01 | 0.09 | ± | 0.03 | 0.07 | ± | 0.02 | ||||
C20:4 n-6 | 0.10 | ± | 0.01 | a | 0.07 | ± | 0.00 | b | 0.10 | ± | 0.01 | a | 0.08 | ± | 0.02 | b |
C20:5 n-3 | 0.04 | ± | 0.01 | b | 0.11 | ± | 0.01 | a | 0.03 | ± | 0.02 | b | 0.10 | ± | 0.02 | a |
C22:5 n-3 | 0.09 | ± | 0.01 | b | 0.12 | ± | 0.00 | ab | 0.08 | ± | 0.07 | b | 0.16 | ± | 0.05 | a |
∑ SCFA(C4 > C8) | 6.58 | ± | 0.50 | a | 6.56 | ± | 0.40 | a | 0.00 | ± | 0.00 | b | 0.00 | ± | 0.00 | b |
∑ MCFA(C10 > C14) | 20.77 | ± | 0.98 | a | 16.15 | ± | 0.43 | c | 18.50 | ± | 0.95 | b | 14.19 | ± | 0.45 | d |
∑ SFA | 77.73 | ± | 1.00 | a | 62.95 | ± | 1.17 | c | 75.25 | ± | 0.74 | b | 58.98 | ± | 0.35 | d |
∑ MUFA | 19.89 | ± | 0.81 | d | 30.29 | ± | 0.93 | b | 21.67 | ± | 0.21 | c | 33.02 | ± | 0.33 | a |
∑ PUFA incl. CLA | 2.38 | ± | 0.19 | d | 6.76 | ± | 0.25 | b | 3.07 | ± | 0.58 | c | 8.01 | ± | 0.38 | a |
∑ PUFA n-3 | 0.48 | ± | 0.05 | c | 1.58 | ± | 0.06 | b | 0.56 | ± | 0.11 | c | 1.83 | ± | 0.05 | a |
∑ PUFA n-6 | 1.26 | ± | 0.10 | b | 1.46 | ± | 0.06 | ab | 1.83 | ± | 0.48 | a | 1.92 | ± | 0.38 | a |
PUFA/SFA | 0.03 | ± | 0.00 | d | 0.11 | ± | 0.01 | b | 0.04 | ± | 0.01 | c | 0.14 | ± | 0.01 | a |
n-6/n-3 | 2.60 | ± | 0.12 | b | 0.93 | ± | 0.01 | c | 3.26 | ± | 0.23 | a | 1.05 | ± | 0.19 | c |
∑ SFA+tFA | 79.78 | ± | 0.86 | a | 70.08 | ± | 0.90 | c | 77.49 | ± | 0.75 | b | 66.96 | ± | 0.30 | d |
∑ C18:1 (t) | 1.74 | ± | 0.13 | c | 6.61 | ± | 0.26 | b | 1.88 | ± | 0.09 | c | 7.28 | ± | 0.07 | a |
C18:1 t9/t11 | 0.35 | ± | 0.01 | a | 0.06 | ± | 0.00 | c | 0.30 | ± | 0.05 | b | 0.06 | ± | 0.00 | c |
∑ CLA | 0.34 | ± | 0.03 | c | 3.19 | ± | 0.13 | b | 0.33 | ± | 0.04 | c | 3.55 | ± | 0.09 | a |
VA + C18:2 c9,t11 | 0.78 | ± | 0.04 | c | 7.31 | ± | 0.26 | b | 0.83 | ± | 0.04 | c | 8.00 | ± | 0.13 | a |
∑ BCFA | 1.75 | ± | 0.05 | d | 2.39 | ± | 0.08 | b | 2.11 | ± | 0.06 | c | 2.80 | ± | 0.03 | a |
FA distribution of milk lipids from TAG (ML conventional and ML Alpine) before preparation (A) to FFA-mixtures suitable for cellular experiments, FA distribution of milk lipids as FFA-mixtures used for incubation of cells after saponification, extraction and solution (B) of milk lipids (FFA) in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.