Table 3.
Control | MLcon | MLalp | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.25% EtOH | 100 μM | 200 μM | 100 μM | 200 μM | ||||||||||||||||
FAME [%] | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||||||||||
C14:0 | 1.58 | ± | 0.39 | d | 6.38 | ± | 0.87 | bc | 7.96 | ± | 0.94 | a | 5.38 | ± | 0.57 | c | 6.81 | ± | 0.51 | ab |
C14:1 c9 | 0.10 | ± | 0.04 | d | 0.24 | ± | 0.05 | bc | 0.33 | ± | 0.05 | a | 0.22 | ± | 0.05 | c | 0.30 | ± | 0.03 | ab |
C15:0 | 0.25 | ± | 0.03 | d | 1.02 | ± | 0.09 | bc | 1.18 | ± | 0.08 | a | 1.08 | ± | 0.09 | c | 1.28 | ± | 0.07 | ab |
C16:0 | 20.92 | ± | 0.95 | d | 34.53 | ± | 1.89 | b | 36.96 | ± | 1.35 | a | 26.52 | ± | 1.14 | c | 26.77 | ± | 0.99 | c |
C16:1 c9 | 12.75 | ± | 0.74 | a | 8.11 | ± | 0.80 | b | 6.64 | ± | 0.52 | c | 7.11 | ± | 0.51 | bc | 5.58 | ± | 0.36 | d |
C16:2 t7,c9 | - | - | - | 0.03 | ± | 0.01 | b | 0.05 | ± | 0.01 | a | |||||||||
C17:0 | 0.44 | ± | 0.03 | c | 0.64 | ± | 0.04 | b | 0.63 | ± | 0.02 | b | 0.69 | ± | 0.04 | a | 0.67 | ± | 0.02 | ab |
C18:0 | 13.02 | ± | 0.47 | a | 10.23 | ± | 0.29 | b | 9.34 | ± | 0.63 | c | 10.13 | ± | 0.37 | b | 8.66 | ± | 0.27 | c |
C18:1 c9 | 34.32 | ± | 0.82 | a | 25.93 | ± | 1.71 | c | 25.02 | ± | 1.66 | c | 29.23 | ± | 1.32 | b | 29.05 | ± | 1.34 | b |
C18:1 c11 | 3.25 | ± | 0.18 | a | 1.85 | ± | 0.24 | b | 1.47 | ± | 0.08 | c | 1.90 | ± | 0.12 | b | 1.56 | ± | 0.07 | c |
C18:1 c12-15 | - | 0.31 | ± | 0.10 | b | 0.39 | ± | 0.09 | ab | 0.42 | ± | 0.13 | ab | 0.48 | ± | 0.11 | a | |||
C18:1 t4-8 | - | 0.03 | ± | 0.01 | bc | 0.03 | ± | 0.01 | bc | 0.08 | ± | 0.06 | ab | 0.11 | ± | 0.05 | a | |||
C18:1 t9 | - | 0.10 | ± | 0.03 | b | 0.10 | ± | 0.04 | b | 0.16 | ± | 0.04 | ab | 0.19 | ± | 0.07 | a | |||
C18:1 t10 | - | 0.14 | ± | 0.04 | b | 0.18 | ± | 0.03 | b | 0.19 | ± | 0.04 | b | 0.25 | ± | 0.05 | a | |||
C18:1 t11 (VA) | - | 0.27 | ± | 0.03 | c | 0.34 | ± | 0.03 | c | 2.50 | ± | 0.20 | b | 3.32 | ± | 0.16 | a | |||
C18:1 t12-16 | - | 0.42 | ± | 0.14 | c | 0.54 | ± | 0.18 | bc | 0.75 | ± | 0.19 | ab | 0.92 | ± | 0.20 | a | |||
C18:2 n-6 | 2.65 | ± | 0.13 | a | 2.08 | ± | 0.05 | cd | 2.04 | ± | 0.17 | d | 2.28 | ± | 0.07 | b | 2.25 | ± | 0.11 | bc |
C18:2 n-6 (t,t) | - | 0.21 | ± | 0.06 | d | 0.29 | ± | 0.03 | c | 0.43 | ± | 0.04 | b | 0.54 | ± | 0.03 | a | |||
C18:2 c9,t11 | 0.09 | ± | 0.01 | e | 0.32 | ± | 0.01 | d | 0.37 | ± | 0.03 | c | 2.47 | ± | 0.07 | b | 3.18 | ± | 0.14 | a |
C18:2 t11,c13 | - | - | - | 0.18 | ± | 0.02 | b | 0.26 | ± | 0.02 | a | |||||||||
C18:2 t9,t11 | - | 0.03 | ± | 0.01 | cd | 0.04 | ± | 0.01 | c | 0.18 | ± | 0.02 | b | 0.24 | ± | 0.03 | a | |||
C18:3 n-3 | 0.22 | ± | 0.03 | d | 0.33 | ± | 0.02 | c | 0.42 | ± | 0.05 | c | 0.96 | ± | 0.02 | b | 1.31 | ± | 0.09 | a |
C20:0 | 0.38 | ± | 0.03 | a | 0.30 | ± | 0.02 | b | 0.24 | ± | 0.03 | c | 0.28 | ± | 0.02 | b | 0.20 | ± | 0.02 | c |
C20:1 c9 | 0.33 | ± | 0.07 | a | 0.17 | ± | 0.04 | bc | 0.15 | ± | 0.02 | d | 0.23 | ± | 0.04 | b | 0.18 | ± | 0.02 | bc |
C20:3 n-6 | 1.19 | ± | 0.14 | a | 0.63 | ± | 0.05 | b | 0.49 | ± | 0.04 | cd | 0.58 | ± | 0.06 | bc | 0.42 | ± | 0.05 | d |
C20:4 n-6 | 2.38 | ± | 0.25 | a | 1.20 | ± | 0.09 | b | 0.86 | ± | 0.05 | c | 1.13 | ± | 0.13 | b | 0.77 | ± | 0.09 | c |
C20:5 n-3 | 0.70 | ± | 0.05 | a | 0.35 | ± | 0.04 | bc | 0.27 | ± | 0.02 | d | 0.39 | ± | 0.03 | b | 0.31 | ± | 0.03 | cd |
C22:5 n-3 | 1.34 | ± | 0.15 | a | 0.70 | ± | 0.11 | b | 0.53 | ± | 0.06 | c | 0.69 | ± | 0.08 | bc | 0.54 | ± | 0.07 | bc |
C22:6 n-3 | 1.76 | ± | 0.16 | a | 0.82 | ± | 0.11 | b | 0.58 | ± | 0.05 | c | 0.78 | ± | 0.10 | b | 0.53 | ± | 0.07 | c |
∑ SFA | 38.65 | ± | 0.88 | a | 55.47 | ± | 2.59 | b | 58.67 | ± | 1.83 | a | 46.78 | ± | 1.59 | c | 47.29 | ± | 1.66 | c |
∑ MUFA | 50.84 | ± | 0.34 | b | 37.69 | ± | 2.28 | c | 35.30 | ± | 1.49 | c | 42.91 | ± | 1.34 | b | 42.07 | ± | 1.22 | b |
∑ PUFA incl. CLA | 10.51 | ± | 0.86 | a | 6.84 | ± | 0.47 | b | 6.03 | ± | 0.44 | b | 10.31 | ± | 0.44 | a | 10.64 | ± | 0.56 | a |
∑ PUFA n-3 | 4.14 | ± | 0.36 | a | 2.31 | ± | 0.28 | c | 1.88 | ± | 0.16 | c | 2.95 | ± | 0.21 | b | 2.82 | ± | 0.22 | b |
∑ PUFA n-6 | 6.28 | ± | 0.51 | a | 3.98 | ± | 0.18 | bc | 3.46 | ± | 0.25 | d | 4.07 | ± | 0.25 | b | 3.54 | ± | 0.23 | cd |
PUFA/SFA | 0.27 | ± | 0.03 | a | 0.12 | ± | 0.01 | c | 0.10 | ± | 0.01 | c | 0.22 | ± | 0.02 | b | 0.23 | ± | 0.02 | b |
n-6/n-3 | 1.52 | ± | 0.06 | b | 1.74 | ± | 0.17 | a | 1.85 | ± | 0.07 | a | 1.38 | ± | 0.03 | bc | 1.26 | ± | 0.02 | c |
∑ SFA + tFA | 38.65 | ± | 0.88 | d | 56.71 | ± | 2.73 | b | 60.23 | ± | 2.06 | a | 50.97 | ± | 2.04 | c | 52.73 | ± | 1.91 | c |
∑ C18:1 (t) | - | 0.96 | ± | 0.18 | c | 1.18 | ± | 0.26 | c | 3.68 | ± | 0.46 | b | 4.79 | ± | 0.38 | a | |||
C18:1 t9,t11 | - | 0.37 | ± | 0.10 | a | 0.28 | ± | 0.09 | a | 0.06 | ± | 0.01 | b | 0.06 | ± | 0.02 | b | |||
∑ CLA | 0.09 | ± | 0.01 | d | 0.35 | ± | 0.02 | c | 0.41 | ± | 0.04 | c | 2.83 | ± | 0.07 | b | 3.68 | ± | 0.15 | a |
VA + C18:2 c9,t11 | 0.09 | ± | 0.01 | d | 0.59 | ± | 0.04 | c | 0.71 | ± | 0.03 | c | 4.97 | ± | 0.25 | b | 6.50 | ± | 0.30 | a |
∑ BCFA | 0.44 | ± | 0.12 | d | 1.59 | ± | 0.15 | c | 1.83 | ± | 0.13 | b | 1.97 | ± | 0.16 | b | 2.38 | ± | 0.14 | a |
FA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.