Table 4.
Control | MLcon | MLalp | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.25% EtOH | 100 μM | 200 μM | 100 μM | 200 μM | ||||||||||||||||
FAME [%] | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||||||||||
C14:0 | 1.67 | ± | 0.32 | d | 6.26 | ± | 0.34 | b | 8.33 | ± | 0.61 | a | 5.36 | ± | 0.28 | c | 6.86 | ± | 0.46 | b |
C14:1 c9 | 0.17 | ± | 0.06 | c | 0.55 | ± | 0.09 | ab | 0.67 | ± | 0.15 | a | 0.46 | ± | 0.06 | b | 0.54 | ± | 0.07 | ab |
C15:0 | 0.21 | ± | 0.01 | c | 0.81 | ± | 0.06 | b | 1.04 | ± | 0.04 | a | 0.85 | ± | 0.04 | b | 1.12 | ± | 0.05 | a |
C16:0 | 20.48 | ± | 0.71 | d | 30.34 | ± | 1.27 | b | 34.36 | ± | 1.34 | a | 24.56 | ± | 0.65 | c | 25.57 | ± | 0.29 | c |
C16:1 c9 | 15.44 | ± | 0.97 | a | 11.82 | ± | 1.23 | b | 9.45 | ± | 1.09 | c | 9.31 | ± | 0.95 | c | 6.96 | ± | 0.73 | d |
C16:2 t7,c9 | - | - | - | 0.07 | ± | 0.01 | b | 0.08 | ± | 0.00 | a | |||||||||
C17:0 | 0.32 | ± | 0.01 | a | 0.54 | ± | 0.04 | c | 0.59 | ± | 0.03 | b | 0.59 | ± | 0.04 | b | 0.68 | ± | 0.03 | d |
C18:0 | 10.97 | ± | 0.89 | a | 9.39 | ± | 0.32 | b | 8.77 | ± | 0.34 | b | 9.03 | ± | 0.21 | b | 8.71 | ± | 0.25 | b |
C18:1 c9 | 36.95 | ± | 0.87 | a | 28.67 | ± | 0.64 | d | 26.09 | ± | 0.27 | e | 31.63 | ± | 0.99 | b | 30.27 | ± | 0.34 | c |
C18:1 c11 | 3.04 | ± | 0.20 | a | 1.77 | ± | 0.15 | b | 1.33 | ± | 0.09 | a | 1.84 | ± | 0.05 | b | 1.39 | ± | 0.11 | a |
C18:1 c12-15 | - | 0.29 | ± | 0.15 | a | 0.38 | ± | 0.07 | a | 0.41 | ± | 0.13 | a | 0.43 | ± | 0.10 | a | |||
C18:1 t4-8 | - | 0.02 | ± | 0.01 | bc | 0.04 | ± | 0.02 | bc | 0.07 | ± | 0.04 | ab | 0.10 | ± | 0.05 | a | |||
C18:1 t9 | - | 0.09 | ± | 0.02 | c | 0.12 | ± | 0.03 | bc | 0.15 | ± | 0.04 | ab | 0.20 | ± | 0.06 | a | |||
C18:1 t10 | - | 0.10 | ± | 0.02 | b | 0.14 | ± | 0.03 | b | 0.14 | ± | 0.04 | b | 0.20 | ± | 0.03 | a | |||
C18:1 t11 (VA) | - | 0.18 | ± | 0.04 | c | 0.28 | ± | 0.05 | c | 1.86 | ± | 0.25 | b | 2.73 | ± | 0.27 | a | |||
C18:1 t12-16 | - | 0.35 | ± | 0.09 | c | 0.58 | ± | 0.08 | b | 0.56 | ± | 0.13 | b | 0.81 | ± | 0.18 | a | |||
C18:2 n-6 | 2.13 | ± | 0.18 | a | 1.91 | ± | 0.12 | bc | 1.73 | ± | 0.07 | c | 2.13 | ± | 0.09 | a | 2.00 | ± | 0.04 | ab |
C18:2 n-6 (t,t) | - | 0.13 | ± | 0.05 | d | 0.22 | ± | 0.03 | c | 0.28 | ± | 0.05 | b | 0.42 | ± | 0.03 | a | |||
C18:2 c9,t11 | 0.07 | ± | 0.02 | d | 0.35 | ± | 0.02 | c | 0.40 | ± | 0.02 | c | 2.90 | ± | 0.27 | b | 3.57 | ± | 0.18 | a |
C18:2 t11,c13 | - | - | - | 0.15 | ± | 0.02 | b | 0.21 | ± | 0.02 | a | |||||||||
C18:2 t9,t11 | - | 0.04 | ± | 0.01 | c | 0.04 | ± | 0.01 | c | 0.19 | ± | 0.02 | b | 0.21 | ± | 0.02 | a | |||
C18:3 n-3 | 0.20 | ± | 0.03 | d | 0.35 | ± | 0.03 | c | 0.39 | ± | 0.03 | c | 1.03 | ± | 0.09 | b | 1.26 | ± | 0.06 | a |
C20:0 | 0.35 | ± | 0.02 | a | 0.30 | ± | 0.02 | b | 0.28 | ± | 0.01 | bc | 0.30 | ± | 0.01 | b | 0.27 | ± | 0.01 | c |
C20:1 c9 | 0.41 | ± | 0.05 | a | 0.19 | ± | 0.04 | cd | 0.15 | ± | 0.00 | d | 0.26 | ± | 0.02 | b | 0.21 | ± | 0.01 | bc |
C20:3 n-6 | 0.97 | ± | 0.12 | a | 0.55 | ± | 0.01 | b | 0.38 | ± | 0.01 | c | 0.53 | ± | 0.02 | b | 0.36 | ± | 0.02 | c |
C20:4 n-6 | 1.82 | ± | 0.19 | a | 1.00 | ± | 0.02 | b | 0.66 | ± | 0.03 | c | 0.95 | ± | 0.04 | b | 0.63 | ± | 0.04 | c |
C20:5 n-3 | 0.53 | ± | 0.05 | a | 0.29 | ± | 0.02 | bc | 0.21 | ± | 0.01 | d | 0.33 | ± | 0.02 | b | 0.25 | ± | 0.01 | cd |
C22:5 n-3 | 1.04 | ± | 0.15 | a | 0.59 | ± | 0.03 | b | 0.42 | ± | 0.01 | c | 0.62 | ± | 0.05 | b | 0.43 | ± | 0.03 | c |
C22:6 n-3 | 1.31 | ± | 0.12 | a | 0.70 | ± | 0.03 | b | 0.45 | ± | 0.03 | c | 0.70 | ± | 0.05 | b | 0.43 | ± | 0.04 | c |
∑ SFA | 35.66 | ± | 1.27 | e | 49.81 | ± | 1.52 | b | 55.66 | ± | 1.15 | a | 43.14 | ± | 1.00 | d | 45.98 | ± | 0.53 | c |
∑ MUFA | 56.10 | ± | 1.15 | a | 44.15 | ± | 1.60 | c | 39.35 | ± | 1.17 | d | 46.84 | ± | 0.86 | b | 43.99 | ± | 0.63 | c |
∑ PUFA incl. CLA | 8.24 | ± | 0.78 | b | 6.04 | ± | 0.20 | c | 4.99 | ± | 0.06 | d | 10.05 | ± | 0.57 | a | 10.03 | ± | 0.17 | a |
∑ PUFA n-3 | 3.20 | ± | 0.34 | a | 2.01 | ± | 0.07 | d | 1.51 | ± | 0.04 | e | 2.78 | ± | 0.17 | b | 2.47 | ± | 0.04 | c |
∑ PUFA n-6 | 4.97 | ± | 0.47 | a | 3.51 | ± | 0.13 | b | 2.82 | ± | 0.06 | c | 3.67 | ± | 0.13 | b | 3.07 | ± | 0.04 | c |
PUFA/SFA | 0.23 | ± | 0.03 | a | 0.12 | ± | 0.00 | b | 0.09 | ± | 0.00 | c | 0.23 | ± | 0.02 | a | 0.22 | ± | 0.00 | a |
n-6/n-3 | 1.56 | ± | 0.07 | c | 1.75 | ± | 0.07 | b | 1.87 | ± | 0.07 | a | 1.32 | ± | 0.03 | d | 1.25 | ± | 0.01 | d |
∑ SFA + tFA | 35.66 | ± | 1.27 | d | 50.75 | ± | 1.62 | b | 57.13 | ± | 1.33 | a | 46.28 | ± | 1.17 | c | 50.56 | ± | 1.04 | b |
∑ C18:1 (t) | - | 0.74 | ± | 0.16 | c | 1.16 | ± | 0.18 | c | 2.78 | ± | 0.45 | b | 4.05 | ± | 0.55 | a | |||
C18:1 t9/t11 | - | 0.51 | ± | 0.07 | a | 0.42 | ± | 0.07 | b | 0.08 | ± | 0.02 | c | 0.07 | ± | 0.02 | c | |||
∑ CLA | 0.07 | ± | 0.02 | d | 0.39 | ± | 0.02 | c | 0.44 | ± | 0.02 | c | 3.26 | ± | 0.33 | b | 3.99 | ± | 0.17 | a |
VA + C18:2 c9,t11 | 0.07 | ± | 0.02 | d | 0.52 | ± | 0.05 | cd | 0.68 | ± | 0.06 | c | 4.75 | ± | 0.52 | b | 6.30 | ± | 0.40 | a |
∑ BCFA | 0.37 | ± | 0.10 | d | 1.36 | ± | 0.13 | c | 1.68 | ± | 0.10 | b | 1.72 | ± | 0.17 | b | 2.16 | ± | 0.11 | a |
FA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.