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. 2011 Aug 4;10:131. doi: 10.1186/1476-511X-10-131

Table 5.

CLA isomers in cellular lipids of HT-29 treated with different milk lipids at 8 h

Control MLcon MLalp

0.25% EtOH 100 μM 200 μM 100 μM 200 μM
CLA FAME [%] Mean SD Mean SD Mean SD Mean SD Mean SD

t12,t14 - - - - -
t11,t13 - 2.63 ± 0.71 a 2.76 ± 0.26 a 1.26 ± 0.14 b 1.31 ± 0.06 b
t10,t12 - 0.69 ± 0.23 a 0.82 ± 0.09 a 0.24 ± 0.09 b 0.26 ± 0.09 b
t9,t11 3.15 ± 1.28 a 3.08 ± 0.49 a 3.35 ± 0.51 a 1.18 ± 0.35 b 1.14 ± 0.34 b
t8,t10 - 0.28 ± 0.15 ab 0.47 ± 0.16 a 0.02 ± 0.01 bc 0.11 ± 0.02 bc
t7,t9 2.86 ± 0.79 a 0.89 ± 0.28 b 0.73 ± 0.08 b 0.09 ± 0.05 b 0.05 ± 0.01 b
trans, trans 6.02 ± 2.06 bc 8.38 ± 1.19 ab 9.68 ± 0.30 a 3.48 ± 0.53 c 3.57 ± 0.50 c
t11,c13 4.88 ± 3.15 ab 3.59 ± 1.20 ab 1.81 ± 0.30 b 6.54 ± 0.47 a 6.83 ± 0.34 a
c11,t13 3.00 ± 1.89 a 1.80 ± 0.93 ab 1.17 ± 0.25 ab 0.31 ± 0.06 b 0.36 ± 0.10 b
t10,c12 3.82 ± 1.39 a 3.48 ± 1.11 b 1.61 ± 0.75 ab 0.42 ± 0.27 b 0.22 ± 0.15 b
c9,t11 77.28 ± 3.25 b 74.65 ± 0.80 a 76.12 ± 0.98 b 86.66 ± 1.17 a 87.14 ± 0.80 a
t8,c10 0.63 ± 0.45 ab 0.78 ± 0.58 ab 1.20 ± 0.25 a 0.44 ± 0.10 ab 0.18 ± 0.10 b
t7,c9 4.38 ± 1.55 bc 6.09 ± 1.58 cd 7.18 ± 0.55 a 1.82 ± 0.20 cd 1.44 ± 0.11 d
cis,trans/trans,cis 93.98 ± 2.06 a 90.39 ± 1.23 b 89.09 ± 0.38 b 96.19 ± 0.61 a 96.17 ± 0.59 a
c11,c13 - - - 0.08 ± 0.02 0.03 ± 0.00
c10,c12 - - - - -
c9,c11 - 1.23 ± 0.10 a 1.18 ± 0.09 a 0.26 ± 0.08 b 0.23 ± 0.09 b
c8,c10 - - - - -
cis/cis - 1.23 ± 0.10 a 1.18 ± 0.09 a 0.34 ± 0.09 b 0.26 ± 0.09 b
t11,c13/t7,c9 1.33 ± 1.25 b 0.64 ± 0.34 b 0.25 ± 0.06 b 3.63 ± 0.46 a 4.77 ± 0.55 a

CLA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.