Table 5.
Control | MLcon | MLalp | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.25% EtOH | 100 μM | 200 μM | 100 μM | 200 μM | ||||||||||||||||
CLA FAME [%] | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||||||||||
t12,t14 | - | - | - | - | - | |||||||||||||||
t11,t13 | - | 2.63 | ± | 0.71 | a | 2.76 | ± | 0.26 | a | 1.26 | ± | 0.14 | b | 1.31 | ± | 0.06 | b | |||
t10,t12 | - | 0.69 | ± | 0.23 | a | 0.82 | ± | 0.09 | a | 0.24 | ± | 0.09 | b | 0.26 | ± | 0.09 | b | |||
t9,t11 | 3.15 | ± | 1.28 | a | 3.08 | ± | 0.49 | a | 3.35 | ± | 0.51 | a | 1.18 | ± | 0.35 | b | 1.14 | ± | 0.34 | b |
t8,t10 | - | 0.28 | ± | 0.15 | ab | 0.47 | ± | 0.16 | a | 0.02 | ± | 0.01 | bc | 0.11 | ± | 0.02 | bc | |||
t7,t9 | 2.86 | ± | 0.79 | a | 0.89 | ± | 0.28 | b | 0.73 | ± | 0.08 | b | 0.09 | ± | 0.05 | b | 0.05 | ± | 0.01 | b |
∑ trans, trans | 6.02 | ± | 2.06 | bc | 8.38 | ± | 1.19 | ab | 9.68 | ± | 0.30 | a | 3.48 | ± | 0.53 | c | 3.57 | ± | 0.50 | c |
t11,c13 | 4.88 | ± | 3.15 | ab | 3.59 | ± | 1.20 | ab | 1.81 | ± | 0.30 | b | 6.54 | ± | 0.47 | a | 6.83 | ± | 0.34 | a |
c11,t13 | 3.00 | ± | 1.89 | a | 1.80 | ± | 0.93 | ab | 1.17 | ± | 0.25 | ab | 0.31 | ± | 0.06 | b | 0.36 | ± | 0.10 | b |
t10,c12 | 3.82 | ± | 1.39 | a | 3.48 | ± | 1.11 | b | 1.61 | ± | 0.75 | ab | 0.42 | ± | 0.27 | b | 0.22 | ± | 0.15 | b |
c9,t11 | 77.28 | ± | 3.25 | b | 74.65 | ± | 0.80 | a | 76.12 | ± | 0.98 | b | 86.66 | ± | 1.17 | a | 87.14 | ± | 0.80 | a |
t8,c10 | 0.63 | ± | 0.45 | ab | 0.78 | ± | 0.58 | ab | 1.20 | ± | 0.25 | a | 0.44 | ± | 0.10 | ab | 0.18 | ± | 0.10 | b |
t7,c9 | 4.38 | ± | 1.55 | bc | 6.09 | ± | 1.58 | cd | 7.18 | ± | 0.55 | a | 1.82 | ± | 0.20 | cd | 1.44 | ± | 0.11 | d |
∑ cis,trans/trans,cis | 93.98 | ± | 2.06 | a | 90.39 | ± | 1.23 | b | 89.09 | ± | 0.38 | b | 96.19 | ± | 0.61 | a | 96.17 | ± | 0.59 | a |
c11,c13 | - | - | - | 0.08 | ± | 0.02 | 0.03 | ± | 0.00 | |||||||||||
c10,c12 | - | - | - | - | - | |||||||||||||||
c9,c11 | - | 1.23 | ± | 0.10 | a | 1.18 | ± | 0.09 | a | 0.26 | ± | 0.08 | b | 0.23 | ± | 0.09 | b | |||
c8,c10 | - | - | - | - | - | |||||||||||||||
∑ cis/cis | - | 1.23 | ± | 0.10 | a | 1.18 | ± | 0.09 | a | 0.34 | ± | 0.09 | b | 0.26 | ± | 0.09 | b | |||
t11,c13/t7,c9 | 1.33 | ± | 1.25 | b | 0.64 | ± | 0.34 | b | 0.25 | ± | 0.06 | b | 3.63 | ± | 0.46 | a | 4.77 | ± | 0.55 | a |
CLA distribution of HT-29 cells after incubation with milk lipids (ML conventional and ML Alpine) given as FFA-mixtures in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.