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. 2000 Feb 1;14(3):349–359.

Figure 1.

Figure 1

Figure 1

Purified human proteins for NER. (A) Incision factors (top line) and repair synthesis factors (bottom line). PCNA, Lig1, and all incision factors are recombinant polypeptides except for TFIIH Hap fr. VI. The XPA, RPA, XPG, PCNA, and Lig1 gels were stained with Coomassie blue and the others with silver. Asterisks represent identified subunits of human RFC, Pol δ, and Pol ε. (B) Recombinant TFIIH. Silver-stained gels (left) and immunoblots (right); equivalent amounts of rIIH6 or rIIH9 were loaded. The six core subunits are present in similar amounts in the two preparations, and the CAK subunits in rIIH9 appear to be roughly stoichiometric with the core subunits.