Skip to main content
. 2000 Feb 1;14(3):349–359.

Figure 2.

Figure 2

Transcription and NER activities of TFIIH copurify. (A) Scheme for TFIIH purification from HeLa cells; TFIIH Hep is the result of step IV, heparin–HPLC and TFIIH Hap is the result of step VI, hydroxyapatite–HPLC. (B) (Top panel) In vitro runoff transcription assays (Tirode et al. 1999), using 1 μl of each fraction; (middle panel) 5 μl of each step V fraction was separated by 10% SDS-PAGE and immunoblotted against the three indicated subunits of TFIIH; (bottom panel) 3 μl of each step V fraction was used in a nucleotide excision repair assay containing all the recombinant proteins needed for dual incision formation (except TFIIH); 1.5 μl of TFIIH Hep fr. IV was used where indicated. Only the area of the gel containing NER excision products is shown. (C) As in B, for step VI TFIIH fractions. (M) 35- and 27-nucleotide molecular weight markers from a labeled MspI digest of pBR322.