Table 1. Characteristics of dsRNA genome segments and proteins of Umatilla virus (USA1969/01).
Seg No. | Seg size (bp) | ORFs bp (including stop codon) | Protein nomenclature (protein structure/putative function) | No. of amino acids | Predicted protein molecular mass (KDa) | G+C content (%) | 5′ non-coding nucleotide sequence of genome segment (positive strand) | 3′ non-coding nucleotide sequence of positive strand (Including stop codon) | Accession Numbers |
1 | 3933 | 13–3912 | VP1 (Pol) | 1299 | 148.135 | 39.05 | 5′–GUUUUAGUUGCC | UAGUCGAGAGGCAACAAAAGAUAC-3′ | HQ842619 |
2 | 2791 | 19–2736 | VP2 (T2) | 905 | 103.654 | 41.53 | 5′–GUUUAAGAUGACUCAGCA | UGAGCACGAGAAAUCAGCCACCUCGCGUUCCUCCUGGAAGGGGGUCAUCAAAAGAUAC-3′ | HQ842620 |
3 | 2411 | 19–2344 | VP3 (Outer cap) | 774 | 90.151 | 38.24 | 5′–GUUUAAAAUCUGAGAGCC | UAAUGUAAGCUUGGCACAGCAAUAUGAAGCCCGUGAUCCUAGUCUUAGGUUCGCUCUCAGGUUAAGGAUAC-3′ | HQ842621 |
4 | 2047 | 30–1772 | NS1 (TuP) | 580 | 66.946 | 40.99 | 5′–GUUUAAAAACCUAUCGGUGUGCCAGCAUC | UGAAAAGAACAUUUUGCAUAACCCACACGAUUAUCAUCAUUUAUCCUUAAUCUUAUUUUUUCGUUUUUAUAAUUAUGUAUUUAGUAAUAGUGUAUGUGUUAGAACAUUUUAUAUCUGUUUUUGUGUAAAAUUAUUAGUAUGUAGAUCAAGAUUAGUGUGUGUACCCGUUUUGUGUGAAAAGCAUUUUAGGCAUAAGAUCCUUAAACCGCUUUGCGAGUGCUCUGUUUGUAUGUAUGUUUCUUGCGUGCAUCUAUAUUAUAGGAUAGGUUUAAGGAUAC-3′ | HQ842622 |
5 | 2021 | 15–1961 | VP4 (Cap) | 648 | 76.048 | 40.03 | 5′–GUUUUAGAUGAACG | UAAAGCGGACUUGCCUCGGGUCGUCGUGCUCACUUUGGGGAGUGUCAUCGUUCACAAGGAUAC-3′ | HQ842623 |
6 | 1681 | 29–1618 | VP5 | 529 | 58.599 | 42.95 | 5′–GUUUAAAAUCUUGCAACGGACCGCAGCC | UAGUUGCCUCUAUCUUCCUAACGAGCUGGACGUGAAAGUCAAGCGGAGACGCAAGAUUAAGGAUAC-3′ | HQ842624 |
7 | 1357 | 80–1303 | NS2 (ViP) | 407 | 45.883 | 43.33 | 5′–GUUUAAAAUCUGACGUACUUUCUGUCACUGGAAGGAGCAUACCAACGAGGAGAAAUCAAGAAAAGUGAAGAGUGAAAAG | UAAAGGACGGCUAGUGUUGUAGUGGGAAGCUCACGAUUCGCGUCAGGUUAAGGAUAC- 3′ | HQ842625 |
8 | 1171 | 18–1073 | VP7 (T13) | 351 | 41.554 | 45.00 | 5′–GUUUUAAAAGCUACAGC | UAAGAGGUAAGGAAGCCCCACACUUUCCCUCUCGCACCCGUGUUGCUCCGCCACGAAGUACCAGGUGUGGGUAUUGAGUGAAUGCUUAGCUUUAAGGAUAC-3′ | HQ842626 |
9 | 1103 | 43–1068 | VP6 (Hel | 341 | 36.471 | 42.70 | 5′–GUUUAAAAUCUGAGUAGCUUUUCAUCCUGAGAGAGUCAUUCA | UAACUCAGUGGCCUGGAGCAACUCGGAUCUCAAGAUAC-3′ | HQ842627 |
10 | 887 | 17–811 | NS3 | 264 | 29.560 | 41.71 | 5′–GUUUAAAAUCUCAAAG | UAGACACUGGAUCGCGGACUGGCACGGUCGAUCUAAGACUACCGGACGUAUGUCGCUACGUCGUUGAGAUUAAGGAUAC-3′ | HQ842628 |
101–811 | NS3a | 236 | 26.422 | 5′–GUUUAAAAUCUCAAAGAUGUUGGAAGCUGCUGAGCGUGCACAAGAACGGAGUAAAGCAACGGAAUCAGAAACGGUUAGAGAAGACAGUGAUAACGAUUCA |
Pol = RNA polymerase; Outer cap = outer capsid protein; Cap = capping enzyme (guanylyltransferase); Hel = helicase enzyme; T2 = protein with T = 2 symmetry; T13 = Protein with T = 13 symmetry; ViP = viral inclusion body matrix protein; TuP = tubule protein. Letters in bold are the stop codon.