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. 2011 Aug 29;6(8):e24111. doi: 10.1371/journal.pone.0024111

Figure 4. Immunochemical demonstration that the N-termini of BmGr8 and BmGr53 are cytoplasmic and the C-termini are extracellular.

Figure 4

BmGr8 was expressed in native form or fused with two MYC-epitopes at its N or C-termini. Cells were processed with mouse anti-MYC and Alexa-labelled anti-mouse antibodies to reveal the accessibility of the MYC antigen under permeabilised or unpermeabilised conditions. (A) Schematic of expression constructs for untagged GRs (controls), N-terminally MYC-tagged GRs (MYC:Grs) and C-terminally MYC-tagged GRs (Grs:MYC). (B) (C) Immunostaining of BmGr8 and BmGr53 in S2 cells under permeabilized and unpermeabilized conditions. Green indicates MYC-directed Alexa immunofluorescence. Blue indicates DAPI nuclear counter stain. Scale Bar  =  5 µm.