Figure 2. p53 and Noxa are increased in TMZ/ABT-737 treated cells.

Immunoblots are of lysates from Annexin V experiments shown in Fig. 1C with 72 h drug exposure. PARP cleavage indicates high levels of apoptosis in combination treated cells. TMZ treatment alone increases levels of p53, but only the combination treatment increases Noxa. Other Bcl-2 family members tested remained unchanged. Relative ratios of Noxa and p53 to tubulin are shown below, with each value normalized to the control.