Figure 4. p53 induction through nutlin-3 causes synergy with ABT-737 in a Noxa-dependent fashion.
(A) Annexin V assays of 1205Lu and A375 cells treated with or without 4 µM nutlin-3 and 3.3 µM ABT-737. (B) A375 shNoxa and shControl cells treated with or without 4 µM nutlin-3 and 3.3 µM ABT-737. (C) Immunoblots of lysates from the Annexin V experiments shown in B show Noxa knockdown and lack of PARP cleavage in the shNoxa line. Relative ratios of Noxa to tubulin are shown below, with each value normalized to the control.